ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (1): 160-164.doi: 10.11843/j.issn.0366-6964.2014.01.023

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Transcription of c-myc mRNA and iNOS mRNA in Central Nervous System in Rats Anesthetized with the Combined Anaesthetic for Miniature Pigs

HOU Jin-long, JIANG Sheng, LI Yan, HU Kui, SONG Xu-dong, NIU Dong-liang, FAN Hong-gang, WANG Hong-bin*   

  1. (College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China)
  • Received:2013-07-18 Online:2014-01-23 Published:2014-01-23

Abstract:

In order to investigate the transcription of c-myc mRNA and iNOS mRNA in central nervous system in rats anesthetized with the Combined
Anaesthetic for Miniature Pigs (named as XFM), 30 rats were divided randomly into two groups: control group and XFM group. XFM group consisted of four subgroups: M1 subgroup (rats received XFM intraperitoneally and then waiting the disappearance of right reflection); M2 subgroup (rats received XFM intraperitoneally and then waiting for 1 h after the disappearance of right reflection); M3 subgroup (rats received XFM intraperitoneally and then waiting the recovery of right reflection) and M4 subgroup (rats received XFM intraperitoneally and then waiting for 1 h after the recovery of right reflection). The brain tissues were taken when reaching each time point. The transcription of c
-myc mRNA and iNOS mRNA in central nervous system was detected with real time PCR. The results show that the transcription of c-myc mRNA in cerebral cortex and thalamus decreased significantly after receiving XFM (P0.01); The transcription of c-myc mRNA in cerebellum, hippocampus and brain stem increased significantly (P0.01). The transcription of iNOS mRNA in all brain regions decreased significantly after receiving XFM (P0.01). These results indicated that XFM could affect the transcription of c-myc mRNA and iNOS mRNA in central nervous system. This might be one of the anaesthetic mechanisms for XFM.

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